 |
|
| Newly developed tissue-engineered biodegradable material for reconstruction of vascular wall without cell seeding |
|
Hiroaki Takahashi1, Mitsuhiro Saito2, Eiichirou Uchimura2, Koujirou Hirakawa3, Eiichi Kaku3, Yutaka Okita1, Yoshiki Sawa2; 1Department of Surgery, Division of Cardiovascular Surgery, Kobe University Graduate School of Medicine, Kobe, Japan; 2Osaka University Graduate School of Medicine, Suita, Japan; 3Senko Medical Ins. Co., Ltd., Tokyo, Japan Objective: Biodegradable materials with autologous cell seeding have attracted much interest as potential cardiovascular grafts. However, the ex vivo cell-seeding pretreatment is complicated, invasive, and can lead to infection. We developed the tissue engineered biodegradable graft material that can promote tissue regeneration without ex vivo cell-seeding to overcome of these obstacles. Methods: The tissue-engineered patch (TEP) was fabricated by compounding a collagen microsponge with biodegradable polymeric scaffold, which was woven with double layer thread composed of polyglycolic acid (PGA) and poly-L-lactic acid (PLLA) [core: PGA, sheath : PLLA]. The TEP (25×20mm) without precellularization were implanted into the canine pulmonary arterial trunk. And no anticoagulants or antiplatelets were administered postoperatively. At 1, 2 and 6 months after implantation (n=4 at each end point), the TEP were explanted and evaluated by histologic, biochemical and immunologic analyses. And reverse transcription-polymerase chain reaction was used to qualify the cellular population in the explanted tissues. For the biochemical examination, a 4-hydroxyproline assay was used to measure the collagen content in the explanted TEPs. The maximal tensile strength of the TEP was measured before implantation and 1,2 and 6 months after implantation with a mechanical tester. Results: There were no sign of thrombus formation on the internal surface of the TEP. Right ventricular angiography showed no evidence of stenosis or aneurismal change. Their luminal surfaces were similar to native arterial tissue. Immunohistological finding showed factor VIII positive endothelial cell monolayer, a parallel alignment of smooth muscle cells at any points after implantation. As for the dry weight collagen content, the difference between the TEP at 6 months after implantation and the native pulmonary arterial wall, was not statistically significant. The quantification of the cell population by polymerase chain reaction showed the vascular endothelial growth factor mRNA expression in the TEPs was higher than that of native pulmonary artery at any points after implantation. The mechanical tensile strength of the TEP before and after implantation was greater than that of the native pulmonary artery. Conclusion: Novel tissue-enginnered patch has the enough potential to accelerate in situ cellularization. This study suggested the patch can be promising as a novel surgical material for the repair of cardiovascular system. Back to 88th Annual Meeting Back to Program Outline |
We Model Excellence |
|
Copyright © American Association for Thoracic Surgery. All rights reserved.
Read the Privacy Policy.
IMPORTANT REMINDER: The preceding information is intended only to provide
general guidance and not as a definitive basis for diagnosis or treatment in any particular case.
It is very important that you consult a doctor about any specific medical problem or question.
Read the Privacy Policy.
IMPORTANT REMINDER: The preceding information is intended only to provide
general guidance and not as a definitive basis for diagnosis or treatment in any particular case.
It is very important that you consult a doctor about any specific medical problem or question.